【(?)产(Chan)品(Pin)简(Jian)介(Jie)】(?)
中(Zhong)文(Wen)名(Ming)称(Cheng)：(?)麻(Ma)疹(Zhen)病(Bing)毒(Du)(MV)检(Jian)测(Ce)试(Shi)剂(Ji)盒(He)
英(Ying)文(Wen)名(Ming)称(Cheng)：(?)Measles virus (MV) ELISA Kit
包(Bao)装(Zhuang)规(Gui)格(Ge)：(?)液(Ye)体(Ti)盒(He)装(Zhuang) 96T/48T
保(Bao)存(Cun)条(Tiao)件(Jian)：(?)2-8℃(?)（(?)不(Bu)使(Shi)用(Yong)时(Shi)，(?)部(Bu)分(Fen)试(Shi)剂(Ji)应(Ying)放(Fang)在(Zai)-20℃(?)条(Tiao)件(Jian)下(Xia)）(?)。(?)
有(You)效(Xiao)期(Qi)：(?)6个(Ge)月(Yue)
存(Cun)储(Chu)运(Yun)输(Shu)：(?)低(Di)温(Wen)避(Bi)光(Guang)，(?)请(Qing)勿(Wu)重(Zhong)压(Ya)，(?)轻(Qing)拿(Na)轻(Qing)放(Fang)（(?)现(Xian)货(Huo)供(Gong)应(Ying)，(?)一(Yi)般(Ban)为(Wei)快(Kuai)递(Di)运(Yun)输(Shu)，(?)江(Jiang)浙(Zhe)沪(Hu)隔(Ge)天(Tian)到(Dao)，(?)外(Wai)地(Di)及(Ji)偏(Pian)远(Yuan)地(Di)方(Fang)三(San)至(Zhi)五(Wu)天(Tian)时(Shi)间(Jian)到(Dao)货(Huo)。(?)）(?)
可(Ke)测(Ce)种(Zhong)属(Shu)：(?)人(Ren)、(?)大(Da)鼠(Shu)、(?)小(Xiao)鼠(Shu)、(?)兔(Tu)子(Zi)、(?)猪(Zhu)、(?)犬(Quan)、(?)猴(Hou)、(?)马(Ma)、(?)牛(Niu)、(?)羊(Yang)、(?)鸡(Ji)、(?)鸭(Ya)、(?)鱼(Yu)等(Deng)。(?)
适(Shi)用(Yong)范(Fan)围(Wei)：(?)此(Ci)试(Shi)剂(Ji)盒(He)仅(Jin)用(Yong)于(Yu)科(Ke)研(Yan)实(Shi)验(Yan)，(?)禁(Jin)用(Yong)于(Yu)临(Lin)床(Chuang)。(?)
性(Xing)能(Neng)优(You)点(Dian)：(?)
1、(?)准(Zhun)确(Que)性(Xing)：(?)标(Biao)准(Zhun)品(Pin)线(Xian)性(Xing)回(Hui)归(Gui)与(Yu)预(Yu)期(Qi)浓(Nong)度(Du)相(Xiang)关(Guan)系(Xi)数(Shu)R值(Zhi)，(?)大(Da)于(Yu)等(Deng)于(Yu)0.9900。(?)
2、(?)灵(Ling)敏(Min)度(Du)：(?)低(Di)检(Jian)测(Ce)浓(Nong)度(Du)小(Xiao)于(Yu)1.0 IU/mL。(?)
3、(?)特(Te)异(Yi)性(Xing)：(?)不(Bu)与(Yu)其(Qi)它(Ta)可(Ke)溶(Rong)性(Xing)结(Jie)构(Gou)类(Lei)似(Si)物(Wu)交(Jiao)叉(Cha)反(Fan)应(Ying)。(?)
4、(?)重(Zhong)复(Fu)性(Xing)：(?)板(Ban)内(Nei)、(?)板(Ban)间(Jian)变(Bian)异(Yi)系(Xi)数(Shu)均(Jun)小(Xiao)于(Yu)15%。(?)
5、(?)贮(Zhu)藏(Cang)：(?)2-8℃(?)，(?)避(Bi)光(Guang)防(Fang)潮(Chao)保(Bao)存(Cun)。(?)
6、(?)有(You)效(Xiao)期(Qi)：(?)6个(Ge)月(Yue)
7、(?)检(Jian)测(Ce)范(Fan)围(Wei)：(?)6.25 IU/mL -200IU/mL
注(Zhu)意(Yi)事(Shi)项(Xiang)：(?)
1、(?)试(Shi)剂(Ji)盒(He)从(Cong)冷(Leng)藏(Cang)环(Huan)境(Jing)中(Zhong)取(Qu)出(Chu)应(Ying)在(Zai)室(Shi)温(Wen)平(Ping)衡(Heng)15-30分(Fen)钟(Zhong)后(Hou)方(Fang)可(Ke)使(Shi)用(Yong)，(?)酶(Mei)标(Biao)包(Bao)被(Bei)板(Ban)开(Kai)封(Feng)后(Hou)如(Ru)未(Wei)用(Yong)完(Wan)，(?)板(Ban)条(Tiao)应(Ying)装(Zhuang)入(Ru)密(Mi)封(Feng)袋(Dai)中(Zhong)保(Bao)存(Cun)。(?)
2、(?)浓(Nong)洗(Xi)涤(Di)液(Ye)可(Ke)能(Neng)会(Hui)有(You)结(Jie)晶(Jing)析(Xi)出(Chu)，(?)稀(Xi)释(Shi)时(Shi)可(Ke)在(Zai)水(Shui)浴(Yu)中(Zhong)加(Jia)温(Wen)助(Zhu)溶(Rong)，(?)洗(Xi)涤(Di)时(Shi)不(Bu)影(Ying)响(Xiang)结(Jie)果(Guo)。(?)
3、(?)各(Ge)步(Bu)加(Jia)样(Yang)均(Jun)应(Ying)使(Shi)用(Yong)加(Jia)样(Yang)器(Qi)，(?)并(Bing)经(Jing)常(Chang)校(Xiao)对(Dui)其(Qi)准(Zhun)确(Que)性(Xing)，(?)以(Yi)避(Bi)免(Mian)试(Shi)验(Yan)误(Wu)差(Cha)。(?)一(Yi)次(Ci)加(Jia)样(Yang)时(Shi)间(Jian)控(Kong)制(Zhi)在(Zai)5分(Fen)钟(Zhong)内(Nei)，(?)如(Ru)标(Biao)本(Ben)数(Shu)量(Liang)多(Duo)，(?)使(Shi)用(Yong)排(Pai)枪(Qiang)加(Jia)样(Yang)。(?)
4、(?)请(Qing)每(Mei)次(Ci)测(Ce)定(Ding)的(De)同(Tong)时(Shi)做(Zuo)标(Biao)准(Zhun)曲(Qu)线(Xian)，(?)做(Zuo)复(Fu)孔(Kong)。(?)如(Ru)标(Biao)本(Ben)中(Zhong)待(Dai)测(Ce)物(Wu)质(Zhi)含(Han)量(Liang)过(Guo)高(Gao)(样(Yang)本(Ben)OD值(Zhi)大(Da)于(Yu)标(Biao)准(Zhun)品(Pin)孔(Kong)*孔(Kong)的(De)OD值(Zhi))，(?)请(Qing)先(Xian)用(Yong)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)稀(Xi)释(Shi)一(Yi)定(Ding)倍(Bei)数(Shu)(n倍(Bei))后(Hou)再(Zai)测(Ce)定(Ding)，(?)计(Ji)算(Suan)时(Shi)请(Qing)zui后(Hou)乘(Cheng)以(Yi)总(Zong)稀(Xi)释(Shi)倍(Bei)数(Shu)(×n×5)。(?)
5、(?)封(Feng)板(Ban)膜(Mo)只(Zhi)限(Xian)一(Yi)次(Ci)性(Xing)使(Shi)用(Yong)，(?)以(Yi)避(Bi)免(Mian)交(Jiao)叉(Cha)污(Wu)染(Ran)。(?)
6、(?)底(Di)物(Wu)请(Qing)避(Bi)光(Guang)保(Bao)存(Cun)。(?)
7、(?)严(Yan)格(Ge)按(An)照(Zhao)说(Shuo)明(Ming)书(Shu)的(De)操(Cao)作(Zuo)进(Jin)行(Xing)，(?)试(Shi)验(Yan)结(Jie)果(Guo)判(Pan)定(Ding)必(Bi)须(Xu)以(Yi)酶(Mei)标(Biao)仪(Yi)读(Du)数(Shu)为(Wei)准(Zhun)。(?)
8、(?)所(Suo)有(You)样(Yang)品(Pin)，(?)洗(Xi)涤(Di)液(Ye)和(He)各(Ge)种(Zhong)废(Fei)弃(Qi)物(Wu)都(Du)应(Ying)按(An)传(Chuan)染(Ran)物(Wu)处(Chu)理(Li)。(?)
9、(?)本(Ben)试(Shi)剂(Ji)不(Bu)同(Tong)批(Pi)号(Hao)组(Zu)分(Fen)不(Bu)得(De)混(Hun)用(Yong)。(?)
10、(?)如(Ru)与(Yu)英(Ying)文(Wen)说(Shuo)明(Ming)书(Shu)有(You)异(Yi)，(?)以(Yi)英(Ying)文(Wen)说(Shuo)明(Ming)书(Shu)为(Wei)准(Zhun)。(?)
操(Cao)作(Zuo)步(Bu)骤(Zhou)：(?)
实(Shi)验(Yan)开(Kai)始(Shi)前(Qian)，(?)请(Qing)提(Ti)前(Qian)配(Pei)置(Zhi)好(Hao)所(Suo)有(You)试(Shi)剂(Ji)，(?)试(Shi)剂(Ji)或(Huo)样(Yang)品(Pin)稀(Xi)释(Shi)时(Shi)，(?)均(Jun)需(Xu)混(Hun)匀(Yun)，(?)混(Hun)匀(Yun)时(Shi)尽(Jin)量(Liang)避(Bi)免(Mian)起(Qi)泡(Pao)。(?)每(Mei)次(Ci)检(Jian)测(Ce)都(Du)应(Ying)该(Gai)做(Zuo)标(Biao)准(Zhun)曲(Qu)线(Xian)。(?)如(Ru)样(Yang)品(Pin)浓(Nong)度(Du)过(Guo)高(Gao)时(Shi)，(?)用(Yong)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)进(Jin)行(Xing)稀(Xi)释(Shi)，(?)以(Yi)使(Shi)样(Yang)品(Pin)符(Fu)合(He)试(Shi)剂(Ji)盒(He)的(De)检(Jian)测(Ce)范(Fan)围(Wei)。(?)
1.         加(Jia)样(Yang)：(?)分(Fen)别(Bie)设(She)空(Kong)白(Bai)孔(Kong)、(?)标(Biao)准(Zhun)孔(Kong)、(?)待(Dai)测(Ce)样(Yang)品(Pin)孔(Kong)。(?)空(Kong)白(Bai)孔(Kong)加(Jia)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)100μl，(?)余(Yu)孔(Kong)分(Fen)别(Bie)加(Jia)标(Biao)准(Zhun)品(Pin)或(Huo)待(Dai)测(Ce)样(Yang)品(Pin)100μl，(?)注(Zhu)意(Yi)不(Bu)要(Yao)有(You)气(Qi)泡(Pao)，(?)加(Jia)样(Yang)将(Jiang)样(Yang)品(Pin)加(Jia)于(Yu)酶(Mei)标(Biao)板(Ban)孔(Kong)底(Di)部(Bu)，(?)尽(Jin)量(Liang)不(Bu)触(Chu)及(Ji)孔(Kong)壁(Bi)，(?)轻(Qing)轻(Qing)晃(Huang)动(Dong)混(Hun)匀(Yun)，(?)酶(Mei)标(Biao)板(Ban)加(Jia)上(Shang)盖(Gai)或(Huo)覆(Fu)膜(Mo)，(?)37℃(?)反(Fan)应(Ying)120分(Fen)钟(Zhong)。(?)为(Wei)保(Bao)证(Zheng)实(Shi)验(Yan)结(Jie)果(Guo)有(You)效(Xiao)性(Xing)，(?)每(Mei)次(Ci)实(Shi)验(Yan)请(Qing)使(Shi)用(Yong)新(Xin)的(De)标(Biao)准(Zhun)品(Pin)溶(Rong)液(Ye)。(?)
2.         弃(Qi)去(Qu)液(Ye)体(Ti)，(?)甩(Shuai)干(Gan)，(?)不(Bu)用(Yong)洗(Xi)涤(Di)。(?)每(Mei)孔(Kong)加(Jia)标(Biao)记(Ji)抗(Kang)体(Ti)工(Gong)作(Zuo)液(Ye)100μl（(?)取(Qu)1μl标(Biao)记(Ji)抗(Kang)体(Ti)加(Jia)99μl标(Biao)记(Ji)抗(Kang)体(Ti)稀(Xi)释(Shi)液(Ye)的(De)比(Bi)例(Li)配(Pei)制(Zhi)，(?)轻(Qing)轻(Qing)混(Hun)匀(Yun)，(?)在(Zai)使(Shi)用(Yong)前(Qian)一(Yi)小(Xiao)时(Shi)内(Nei)配(Pei)制(Zhi)）(?)，(?)37℃(?),60分(Fen)钟(Zhong)。(?)
3.         温(Wen)育(Yu)60分(Fen)钟(Zhong)后(Hou)，(?)弃(Qi)去(Qu)孔(Kong)内(Nei)液(Ye)体(Ti)，(?)甩(Shuai)干(Gan)，(?)洗(Xi)板(Ban)3次(Ci)，(?)每(Mei)次(Ci)浸(Jin)泡(Pao)1-2分(Fen)钟(Zhong)，(?)350μl/每(Mei)孔(Kong)，(?)甩(Shuai)干(Gan)。(?)
4.         每(Mei)孔(Kong)加(Jia)辣(La)根(Gen)过(Guo)氧(Yang)化(Hua)物(Wu)酶(Mei)标(Biao)记(Ji)亲(Qin)和(He)素(Su)工(Gong)作(Zuo)液(Ye)（(?)同(Tong)标(Biao)记(Ji)抗(Kang)体(Ti)工(Gong)作(Zuo)液(Ye)）(?)100μl，(?)37℃(?)，(?)60分(Fen)钟(Zhong)。(?)
5.         温(Wen)育(Yu)60分(Fen)钟(Zhong)后(Hou)，(?)弃(Qi)去(Qu)孔(Kong)内(Nei)液(Ye)体(Ti)，(?)甩(Shuai)干(Gan)，(?)洗(Xi)板(Ban)5次(Ci)，(?)每(Mei)次(Ci)浸(Jin)泡(Pao)1-2分(Fen)钟(Zhong)，(?)350μl/每(Mei)孔(Kong)，(?)甩(Shuai)干(Gan)。(?)
6.         依(Yi)序(Xu)每(Mei)孔(Kong)加(Jia)底(Di)物(Wu)溶(Rong)液(Ye)90μl，(?)37℃(?)避(Bi)光(Guang)显(Xian)色(Se)（(?)30分(Fen)钟(Zhong)内(Nei)，(?)此(Ci)时(Shi)肉(Rou)眼(Yan)可(Ke)见(Jian)标(Biao)准(Zhun)品(Pin)的(De)前(Qian)3-4孔(Kong)有(You)明(Ming)显(Xian)的(De)梯(Ti)度(Du)蓝(Lan)色(Se)，(?)后(Hou)3-4孔(Kong)梯(Ti)度(Du)不(Bu)明(Ming)显(Xian)，(?)即(Ji)可(Ke)终(Zhong)止(Zhi)）(?)。(?)
7.         依(Yi)序(Xu)每(Mei)孔(Kong)加(Jia)终(Zhong)止(Zhi)溶(Rong)液(Ye)50μl，(?)终(Zhong)止(Zhi)反(Fan)应(Ying)（(?)此(Ci)时(Shi)蓝(Lan)色(Se)立(Li)转(Zhuan)黄(Huang)色(Se)）(?)。(?)终(Zhong)止(Zhi)液(Ye)的(De)加(Jia)入(Ru)顺(Shun)序(Xu)应(Ying)尽(Jin)量(Liang)与(Yu)底(Di)物(Wu)液(Ye)的(De)加(Jia)入(Ru)顺(Shun)序(Xu)相(Xiang)同(Tong)。(?)为(Wei)了(Liao)保(Bao)证(Zheng)实(Shi)验(Yan)结(Jie)果(Guo)的(De)准(Zhun)确(Que)性(Xing)，(?)底(Di)物(Wu)反(Fan)应(Ying)时(Shi)间(Jian)到(Dao)后(Hou)应(Ying)尽(Jin)快(Kuai)加(Jia)入(Ru)终(Zhong)止(Zhi)液(Ye)。(?)
8.         用(Yong)酶(Mei)联(Lian)仪(Yi)在(Zai)450nm波(Bo)长(Chang)依(Yi)序(Xu)测(Ce)量(Liang)各(Ge)孔(Kong)的(De)光(Guang)密(Mi)度(Du)（(?)OD值(Zhi)）(?)。(?) 在(Zai)加(Jia)终(Zhong)止(Zhi)液(Ye)后(Hou)15分(Fen)钟(Zhong)以(Yi)内(Nei)进(Jin)行(Xing)检(Jian)测(Ce)。(?)
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金(Jin)龟(Gui)子(Zi)绿(Lu)僵(Jiang)菌(Jun)Human TNFRSF10B(Tumor necrosis factor receptor superfamily member 10B) ELISA Kit用(Yong)途(Tu): 生(Sheng)物(Wu)防(Fang)治(Zhi)

死(Si)亡(Wang)谷(Gu)芽(Ya)孢(Bao)杆(Gan)菌(Jun)Human PLA2G2A(Phospholipase A2, membrane associated) ELISA Kit拉(La)丁(Ding)属(Shu)名(Ming): Bacillus  vallismortis

传(Chuan)染(Ran)性(Xing)法(Fa)氏(Shi)囊(Nang)病(Bing)病(Bing)Human IK(Protein Red) ELISA Kit拉(La)丁(Ding)属(Shu)名(Ming): Infectious       bursal disease virus

慢(Man)生(Sheng)根(Gen)瘤(Liu)菌(Jun)Human FCER1A(High affinity immunoglobulin epsilon receptor subunit alpha) ELISA Kit用(Yong)途(Tu): 与(Yu)胡(Hu)枝(Zhi)子(Zi)共(Gong)生(Sheng)固(Gu)氮(Dan)

坚(Jian)强(Qiang)芽(Ya)孢(Bao)杆(Gan)菌(Jun)Human BIN1(Myc box-dependent-interacting protein 1) ELISA Kit拉(La)丁(Ding)属(Shu)名(Ming): Bacillus firmus

鸡(Ji)伤(Shang)寒(Han)沙(Sha)门(Men)氏(Shi)菌(Jun)Human FUT3(Galactoside 3(4)-L-fucosyltransferase) ELISA Kit拉(La)丁(Ding)属(Shu)名(Ming): gallinarum

嗜(Shi)果(Guo)刀(Dao)孢(Bao)Human HSF4(Heat shock factor protein 4) ELISA Kit仅(Jin)用(Yong)于(Yu)科(Ke)学(Xue)研(Yan)究(Jiu)或(Huo)者(Zhe)工(Gong)业(Ye)应(Ying)用(Yong)等(Deng)非(Fei)医(Yi)疗(Liao)目(Mu)的(De)，(?)不(Bu)可(Ke)用(Yong)于(Yu)人(Ren)类(Lei)或(Huo)动(Dong)物(Wu)的(De)临(Lin)床(Chuang)诊(Zhen)断(Duan)或(Huo)治(Zhi)疗(Liao)，(?)非(Fei)药(Yao)用(Yong)，(?)非(Fei)食(Shi)用(Yong)
麻(Ma)疹(Zhen)病(Bing)毒(Du)(MV)检(Jian)测(Ce)试(Shi)剂(Ji)盒(He)趋(Qu)化(Hua)样(Yang)因(Yin)子(Zi)受(Shou)体(Ti)1抗(Kang)体(Ti)6-氟(Fu)吲(Yin)哚(Duo)Porcine PCDHγA2 (Protocadherin Gamma A2)  

造(Zao)血(Xue)干(Gan)抗(Kang)原(Yuan)CD133抗(Kang)体(Ti)6-氟(Fu)吲(Yin)哚(Duo)Porcine IGF-1 (Insulin Like Growth Factor 1)  

G蛋(Dan)白(Bai)偶(Ou)联(Lian)受(Shou)体(Ti)相(Xiang)互(Hu)作(Zuo)用(Yong)蛋(Dan)白(Bai)1抗(Kang)体(Ti)（(?)补(Bu)体(Ti)Clq TNF相(Xiang)关(Guan)蛋(Dan)白(Bai)1）(?)5-氟(Fu)吲(Yin)哚(Duo)Porcine AP13 (Apelin 13)

心(Xin)肌(Ji)营(Ying)养(Yang)素(Su)1抗(Kang)体(Ti)5-氟(Fu)吲(Yin)哚(Duo)Porcine SPJ (Surfactant Associated Protein J)

C型(Xing)凝(Ning)集(Ji)素(Su)结(Jie)构(Gou)域(Yu)家(Jia)族(Zu)1成(Cheng)员(Yuan)B抗(Kang)体(Ti)5-氟(Fu)吲(Yin)哚(Duo)Porcine ACE2 (Angiotensin I Converting Enzyme 2)

阳(Yang)离(Li)子(Zi)通(Tong)道(Dao)相(Xiang)关(Guan)蛋(Dan)白(Bai)β亚(Ya)基(Ji)抗(Kang)体(Ti)5-氟(Fu)-2-甲(Jia)基(Ji)吲(Yin)哚(Duo)Porcine ACD (Adrenocortical Dysplasia Homolog)

特(Te)异(Yi)性(Xing)蛋(Dan)白(Bai)57抗(Kang)体(Ti)5-氟(Fu)吲(Yin)哚(Duo)-2-甲(Jia)酸(Suan)Porcine FGF23 (Fibroblast Growth Factor 23)

L选(Xuan)择(Ze)素(Su)抗(Kang)体(Ti)5-氟(Fu)吲(Yin)哚(Duo)-2-甲(Jia)酸(Suan)Porcine THRα (Thyroid Hormone Receptor Alpha)