产(Chan)品(Pin)及(Ji)特(Te)点(Dian):(?)
荧(Ying)光(Guang)定(Ding)量(Liang)PCR是(Shi)在(Zai)PCR体(Ti)系(Xi)中(Zhong)加(Jia)入(Ru)荧(Ying)光(Guang)化(Hua)合(He)物(Wu)(荧(Ying)光(Guang)染(Ran)料(Liao)或(Huo)荧(Ying)光(Guang)探(Tan)针(Zhen))。(?)利(Li)用(Yong)荧(Ying)光(Guang)信(Xin)号(Hao)积(Ji)累(Lei)实(Shi)时(Shi)监(Jian)测(Ce)整(Zheng)个(Ge)PCR进(Jin)程(Cheng),(?)使(Shi)每(Mei)个(Ge)循(Xun)环(Huan)变(Bian)得(De)“可(Ke)见(Jian)”。(?)本(Ben)产(Chan)品(Pin)就(Jiu)是(Shi)以(Yi)探(Tan)针(Zhen)法(Fa)荧(Ying)光(Guang)定(Ding)量(Liang) RT-PCR 技(Ji)术(Shu)为(Wei)基(Ji)础(Chu)开(Kai)发(Fa)的(De)专(Zhuan)门(Men)检(Jian)测(Ce)探(Tan)针(Zhen)法(Fa)荧(Ying)光(Guang)定(Ding)量(Liang)PCR试(Shi)剂(Ji)盒(He)的(De)试(Shi)剂(Ji)盒(He)。(?)
产(Chan)品(Pin)名(Ming)称(Cheng) | 规(Gui)格(Ge) | 货(Huo)号(Hao) |
大(Da)鼠(Shu)源(Yuan)性(Xing)成(Cheng)分(Fen)探(Tan)针(Zhen)法(Fa)PCR荧(Ying)光(Guang)定(Ding)量(Liang)试(Shi)剂(Ji)盒(He) | 50次(Ci) | EY00P9519 |
运(Yun)输(Shu)及(Ji)保(Bao)存(Cun):(?) 低(Di)温(Wen)运(Yun)输(Shu),(?)-20℃(?)保(Bao)存(Cun),(?)保(Bao)存(Cun)期(Qi)限(Xian)为(Wei)12个(Ge)月(Yue)。(?)
自(Zi)备(Bei)试(Shi)剂(Ji):(?)样(Yang)品(Pin) RNA。(?)
分(Fen)类(Lei):(?)荧(Ying)光(Guang)定(Ding)量(Liang)PCR试(Shi)剂(Ji)盒(He)
储(Chu)存(Cun)条(Tiao)件(Jian):(?)-20℃(?)避(Bi)光(Guang)保(Bao)存(Cun),(?)避(Bi)免(Mian)反(Fan)复(Fu)冻(Dong)融(Rong)。(?)
运(Yun)输(Shu):(?)低(Di)温(Wen)、(?)避(Bi)光(Guang),(?)快(Kuai)递(Di)免(Mian)费(Fei)。(?)
客(Ke)户(Hu)只(Zhi)需(Xu)提(Ti)供(Gong)样(Yang)品(Pin)和(He)待(Dai)检(Jian)测(Ce)基(Ji)因(Yin)名(Ming)称(Cheng)即(Ji)可(Ke)。(?)由(You)我(Wo)们(Men)提(Ti)取(Qu)RNA,(?)设(She)计(Ji)并(Bing)合(He)成(Cheng)引(Yin)物(Wu),(?)完(Wan)成(Cheng)荧(Ying)光(Guang)定(Ding)量(Liang)PCR实(Shi)验(Yan)以(Yi)及(Ji)后(Hou)续(Xu)数(Shu)据(Ju)分(Fen)析(Xi),(?)提(Ti)供(Gong)实(Shi)验(Yan)报(Bao)告(Gao)给(Gei)您(Nin)。(?)
染(Ran)料(Liao)法(Fa)
探(Tan)针(Zhen)法(Fa) (?)
可(Ke)进(Jin)行(Xing)相(Xiang)对(Dui),(?)定(Ding)量(Liang)表(Biao)达(Da)分(Fen)析(Xi),(?)定(Ding)性(Xing)分(Fen)析(Xi),(?)SNP检(Jian)测(Ce)等(Deng)
实(Shi)验(Yan)报(Bao)告(Gao)
电(Dian)泳(Yong)图(Tu) ·标(Biao)准(Zhun)曲(Qu)线(Xian)
溶(Rong)解(Jie)曲(Qu)线(Xian) ·扩(Kuo)增(Zeng)曲(Qu)线(Xian)
完(Wan)整(Zheng)的(De)实(Shi)验(Yan)过(Guo)程(Cheng)、(?)试(Shi)剂(Ji)、(?)仪(Yi)器(Qi)及(Ji)分(Fen)析(Xi)报(Bao)告(Gao)
使(Shi)用(Yong)方(Fang)法(Fa)
一(Yi)、(?)稀(Xi)释(Shi)标(Biao)准(Zhun)曲(Qu)线(Xian)样(Yang)品(Pin)((?)以(Yi) 10E2-10E7 拷(Kao)贝(Bei)/μL 这(Zhe) 6 个(Ge) 10 倍(Bei)稀(Xi)释(Shi)度(Du)为(Wei)例(Li))(?)。(?)由(You)于(Yu)标(Biao)准(Zhun)品(Pin)浓(Nong)度(Du)非(Fei)常(Chang)高(Gao),(?)因(Yin)此(Ci)下(Xia)列(Lie)稀(Xi)释(Shi)操(Cao)作(Zuo)一(Yi)定(Ding)要(Yao)在(Zai)独(Du)立(Li)的(De)区(Qu)域(Yu)进(Jin)行(Xing),(?)千(Qian)万(Wan)不(Bu)能(Neng)污(Wu)染(Ran)样(Yang)品(Pin)或(Huo)本(Ben)试(Shi)剂(Ji)盒(He)的(De)其(Qi)他(Ta)成(Cheng)分(Fen))(?)。(?)为(Wei)增(Zeng)加(Jia)产(Chan)品(Pin)稳(Wen)定(Ding)性(Xing)和(He)避(Bi)免(Mian)扩(Kuo)散(San)传(Chuan)染(Ran)性(Xing)病(Bing)原(Yuan),(?)本(Ben)产(Chan)品(Pin)不(Bu)提(Ti)供(Gong)活(Huo)体(Ti)样(Yang)品(Pin)做(Zuo)阳(Yang)性(Xing)对(Dui)照(Zhao),(?)只(Zhi)提(Ti)供(Gong)无(Wu)传(Chuan)染(Ran)性(Xing)的(De) DNA 段(Duan)作(Zuo)为(Wei)阳(Yang)性(Xing)对(Dui)照(Zhao)。(?)
1. 标(Biao)记(Ji) 6 个(Ge)离(Li)心(Xin)管(Guan),(?)分(Fen)别(Bie)为(Wei) 7,(?)6,(?)5,(?)4,(?)3,(?)2。(?)
2. 用(Yong)带(Dai)芯(Xin)枪(Qiang)头(Tou)分(Fen)别(Bie)加(Jia)入(Ru) 45 μL 荧(Ying)光(Guang) RT-PCR 模(Mo)板(Ban)稀(Xi)释(Shi)液(Ye),(?)用(Yong)带(Dai)芯(Xin)枪(Qiang)头(Tou),(?)下(Xia)同(Tong))(?)。(?)
3. 在(Zai) 7 号(Hao)管(Guan)中(Zhong)加(Jia)入(Ru) 5 μL 1×10E8 拷(Kao)贝(Bei)/μL 的(De)阳(Yang)性(Xing)对(Dui)照(Zhao)(试(Shi)剂(Ji)盒(He)提(Ti)供(Gong)),(?)充(Chong)分(Fen)震(Zhen)荡(Dang)1分(Fen)钟(Zhong),(?)得(De) 1×10E7 拷(Kao)贝(Bei)/μL 的(De)标(Biao)准(Zhun)曲(Qu)线(Xian)样(Yang)品(Pin)。(?)放(Fang)冰(Bing)上(Shang)待(Dai)用(Yong)。(?)
4. 换(Huan)枪(Qiang)头(Tou),(?)在(Zai) 6 号(Hao)管(Guan)中(Zhong)加(Jia)入(Ru) 5 μL 1×10E7 拷(Kao)贝(Bei)/μL 的(De)阳(Yang)性(Xing)对(Dui)照(Zhao)(上(Shang)步(Bu)稀(Xi)释(Shi)所(Suo)得(De)),(?)充(Chong)分(Fen)震(Zhen)荡(Dang) 1 分(Fen)钟(Zhong),(?)得(De) 1×10E6 拷(Kao)贝(Bei)/μL 的(De)标(Biao)准(Zhun)曲(Qu)线(Xian)样(Yang)品(Pin)。(?)放(Fang)冰(Bing)上(Shang)待(Dai)用(Yong)。(?)
5. 换(Huan)枪(Qiang)头(Tou),(?)在(Zai) 5 号(Hao)管(Guan)中(Zhong)加(Jia)入(Ru) 5 μL 1×10E6 拷(Kao)贝(Bei)/μL 的(De)阳(Yang)性(Xing)对(Dui)照(Zhao)(上(Shang)步(Bu)稀(Xi)释(Shi)所(Suo)得(De)),(?)充(Chong)分(Fen)震(Zhen)荡(Dang)1分(Fen)钟(Zhong),(?)得(De) 1×10E5 拷(Kao)贝(Bei)/μL 的(De)标(Biao)准(Zhun)曲(Qu)线(Xian)样(Yang)品(Pin)。(?)放(Fang)冰(Bing)上(Shang)待(Dai)用(Yong)。(?)
6. 重(Zhong)复(Fu)上(Shang)面(Mian)的(De)操(Cao)作(Zuo)直(Zhi)到(Dao)得(De)到(Dao) 6 个(Ge)稀(Xi)释(Shi)度(Du)的(De)标(Biao)准(Zhun)曲(Qu)线(Xian)样(Yang)品(Pin)。(?)放(Fang)冰(Bing)上(Shang)待(Dai)用(Yong)。(?)
二(?)、(?)样(Yang)品(Pin) RNA 的(De)制(Zhi)备(Bei)
7. 如(Ru)果(Guo)有(You)N个(Ge)样(Yang)品(Pin),(?)设(She)置(Zhi)N+2个(Ge)提(Ti)取(Qu),(?)多(Duo)出(Chu)的(De)一(Yi)个(Ge)是(Shi)PC((?)样(Yang)品(Pin)制(Zhi)备(Bei)阳(Yang)性(Xing)对(Dui)照(Zhao))(?),(?)一(Yi)个(Ge)是(Shi) NC((?)样(Yang)品(Pin)制(Zhi)备(Bei)阴(Yin)性(Xing)对(Dui)照(Zhao))(?)。(?)可(Ke)以(Yi)用(Yong)10μL阳(Yang)性(Xing)对(Dui)照(Zhao)的(De)10000倍(Bei)稀(Xi)释(Shi)液(Ye)再(Zai)加(Jia)上(Shang)一(Yi)定(Ding)量(Liang)的(De)水(Shui)使(Shi)总(Zong)体(Ti)积(Ji)跟(Gen)每(Mei)次(Ci)制(Zhi)备(Bei)要(Yao)求(Qiu)的(De)体(Ti)积(Ji)一(Yi)样(Yang),(?)以(Yi)此(Ci)作(Zuo)为(Wei)PC。(?)另(Ling)外(Wai)用(Yong)水(Shui)作(Zuo)为(Wei)NC。(?)
8. 用(Yong)自(Zi)选(Xuan)方(Fang)法(Fa)纯(Chun)化(Hua)样(Yang)品(Pin)的(De)RNA,(?)本(Ben)试(Shi)剂(Ji)盒(He)跟(Gen)市(Shi)场(Chang)上(Shang)大(Da)多(Duo)数(Shu)病(Bing)毒(Du)RNA提(Ti)取(Qu)试(Shi)剂(Ji)盒(He)兼(Jian)容(Rong)。(?)也(Ye)以(Yi)选(Xuan)购(Gou)本(Ben)公(Gong)司(Si)的(De)柱(Zhu)式(Shi)病(Bing)毒(Du)RNAout。(?)本(Ben)试(Shi)剂(Ji)盒(He)免(Mian)费(Fei)赠(Zeng)送(Song)20次(Ci)免(Mian)RNA提(Ti)取(Qu)的(De)核(He)酸(Suan)释(Shi)放(Fang)剂(Ji)试(Shi)用(Yong)装(Zhuang),(?)该(Gai)释(Shi)放(Fang)剂(Ji)的(De)裂(Lie)解(Jie)液(Ye)可(Ke)以(Yi)直(Zhi)接(Jie)作(Zuo)为(Wei)RT-PCR模(Mo)板(Ban),(?)省(Sheng)略(Lue)了(Liao)RNA提(Ti)取(Qu)步(Bu)骤(Zhou)。(?)
三(San)、(?)Probe qRT-PCR 反(Fan)应(Ying)((?)20μL 体(Ti)系(Xi),(?)在(Zai)样(Yang)品(Pin)制(Zhi)备(Bei)室(Shi)进(Jin)行(Xing))(?)
9. 如(Ru)果(Guo)做(Zuo)定(Ding)量(Liang)分(Fen)析(Xi)并(Bing)且(Qie)只(Zhi)做(Zuo)1次(Ci)重(Zhong)复(Fu),(?)则(Ze)标(Biao)记(Ji)N+9个(Ge)PCR 管(Guan),(?)其(Qi)中(Zhong)N+2个(Ge)用(Yong)于(Yu)上(Shang)步(Bu)得(De)到(Dao)的(De) N+2个(Ge)样(Yang)品(Pin),(?)1个(Ge)用(Yong)于(Yu)PCR阴(Yin)性(Xing)对(Dui)照(Zhao)((?)用(Yong)水(Shui)做(Zuo)模(Mo)板(Ban))(?),(?)6个(Ge)用(Yong)于(Yu)标(Biao)准(Zhun)曲(Qu)线(Xian)。(?)如(Ru)果(Guo)做(Zuo)定(Ding)性(Xing)分(Fen)析(Xi),(?)并(Bing)且(Qie)只(Zhi)做(Zuo)1次(Ci)重(Zhong)复(Fu),(?)则(Ze)标(Biao)记(Ji)N+4 个(Ge)PCR管(Guan),(?)其(Qi)中(Zhong)N+2个(Ge)用(Yong)于(Yu)上(Shang)步(Bu)得(De)到(Dao)的(De)N+2个(Ge)样(Yang)品(Pin),(?)1 个(Ge)用(Yong)于(Yu) PCR 阴(Yin)性(Xing)对(Dui)照(Zhao)((?)用(Yong)水(Shui)做(Zuo)模(Mo)板(Ban))(?),(?)1个(Ge)用(Yong)于(Yu)PCR阳(Yang)性(Xing)对(Dui)照(Zhao)((?)用(Yong)第(Di)4号(Hao)管(Guan)的(De)阳(Yang)性(Xing)对(Dui)照(Zhao)稀(Xi)释(Shi)液(Ye)做(Zuo)模(Mo)板(Ban))(?)。(?)下(Xia)面(Mian)只(Zhi)以(Yi)定(Ding)量(Liang)分(Fen)析(Xi)为(Wei)例(Li)描(Miao)述(Shu)操(Cao)作(Zuo)步(Bu)骤(Zhou)。(?)
10. 在(Zai)标(Biao)记(Ji)管(Guan)中(Zhong)按(An)下(Xia)表(Biao)加(Jia)入(Ru)各(Ge)成(Cheng)分(Fen)((?)本(Ben)表(Biao)只(Zhi)列(Lie)出(Chu)一(Yi)次(Ci)重(Zhong)复(Fu)。(?)样(Yang)品(Pin)管(Guan)和(He)阴(Yin)性(Xing)对(Dui)照(Zhao)设(She)置(Zhi)完(Wan)毕(Bi)后(Hou)才(Cai)设(She)置(Zhi)阳(Yang)性(Xing)对(Dui)照(Zhao),(?)并(Bing)且(Qie)阳(Yang)性(Xing)对(Dui)照(Zhao)样(Yang)品(Pin)要(Yao)等(Deng)所(Suo)有(You)管(Guan)子(Zi)盖(Gai)上(Shang)盖(Gai)子(Zi)储(Chu)存(Cun)好(Hao)后(Hou)加(Jia))(?)
谷(Gu)蛋(Dan)白(Bai)外(Wai)啡(Fei)肽(Tai)B5BOC-L- 97%Human VN1R4 ELISA Kit
谷(Gu)蛋(Dan)白(Bai)外(Wai)啡(Fei)肽(Tai)CBoc-L- 98%Human VMO1 ELISA Kit
糖(Tang)原(Yuan)合(He)成(Cheng)酶(Mei)导(Dao)出(Chu)肽(Tai)BOC-D- 98%Human VNN2 ELISA Kit
糖(Tang)蛋(Dan)白(Bai)激(Ji)素(Su)a(32-46)酰(Xian)BOC-L-羟(Qiang)甲(Jia)酯(Zhi) 98%Human VPRBP ELISA Kit
糖(Tang)蛋(Dan)白(Bai)Ⅱ(?)b片(Pian)段(Duan)((?)300-312)(?)BOC-L-苯(Ben) 98%Human VBP1 ELISA Kit
糖(Tang)蛋(Dan)白(Bai)Ⅱ(?)b片(Pian)段(Duan)((?)656-667)(?)BOC-D-天(Tian)冬(Dong)酰(Xian) 98%Human VCP(valosin-containing protein) ELISA Kit
粒(Li)细(Xi)胞(Bao)巨(Ju)噬(Shi)细(Xi)胞(Bao)激(Ji)因(Yin)子(Zi)((?)17-31)(?)BOC-L-苯(Ben)甘(Gan)氨(An) 98%Human VPREB3 ELISA Kit
粒(Li)细(Xi)胞(Bao)巨(Ju)噬(Shi)细(Xi)胞(Bao)激(Ji)因(Yin)子(Zi)((?)96-112)(?)BOC-D- 98%Human VDAC3 ELISA Kit
GMAP (16-41)酰(Xian)BOC-L-甲(Jia)酯(Zhi) 99%Human CNDP2(Cytosolic non-specific dipeptidase) ELISA Kit
GMAP (25-41), 酰(Xian)BOC-L-羟(Qiang) 98%Human VDAC2 ELISA Kit
释(Shi)放(Fang)激(Ji)素(Su)相(Xiang)关(Guan)蛋(Dan)白(Bai)((?)1-13)(?),(?)人(Ren)BOC-L-脯(Fu)氨(An) 98%Human VCPIP1 ELISA Kit
促(Cu)性(Xing)性(Xing)激(Ji)素(Su)T-V((?)nt38-67)(?)BOC-L- 98%Human VEZT ELISA Kit
gp38BOC-L-天(Tian)冬(Dong)酰(Xian) BRHuman VGLL4 ELISA Kit
gp100 (177-186)BOC-L-甲(Jia)酯(Zhi) BRHuman CNDP1(Beta-Ala-His dipeptidase) ELISA Kit
gp100 (178 - 187)BOC-D- BRHuman VILIP 3 ELISA Kit
大(Da)鼠(Shu)源(Yuan)性(Xing)成(Cheng)分(Fen)探(Tan)针(Zhen)法(Fa)PCR荧(Ying)光(Guang)定(Ding)量(Liang)试(Shi)剂(Ji)盒(He)蛋(Dan)白(Bai)O-甘(Gan)露(Lu)糖(Tang)基(Ji)转(Zhuan)移(Yi)酶(Mei)2(POMT2)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))标(Biao)准(Zhun)溶(Rong)液(Ye)(100μg/ml,u=3%,基(Ji)体(Ti):酮(Tong)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>98.0%(HPLC)(T)
外(Wai)周(Zhou)蛋(Dan)白(Bai)(PRPH)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))死(Si)蜱(Pi)(标(Biao)准(Zhun)品(Pin)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>97.0%(HPLC)(T)
过(Guo)氧(Yang)化(Hua)还(Huan)原(Yuan)酶(Mei)3(PRDX3)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))死(Si)蜱(Pi)标(Biao)准(Zhun)溶(Rong)液(Ye)(10μg/ml,u=6~(?)3%,酮(Tong)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>98.0%(GC)(T)
过(Guo)氧(Yang)化(Hua)还(Huan)原(Yuan)酶(Mei)4(PRDX4)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))高(Gao)效(Xiao)氯(Lu)氟(Fu)菊(Ju)酯(Zhi)(标(Biao)准(Zhun)品(Pin)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>95.0%(T)
过(Guo)氧(Yang)化(Hua)还(Huan)原(Yuan)酶(Mei)6(PRDX6)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))高(Gao)效(Xiao)氯(Lu)氟(Fu)菊(Ju)酯(Zhi)标(Biao)准(Zhun)溶(Rong)液(Ye)(100μg/ml,u=2%,基(Ji)体(Ti):石(Shi)油(You)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>98.0%(GC)
核(He)糖(Tang)体(Ti)结(Jie)合(He)糖(Tang)蛋(Dan)白(Bai)Ⅱ(?)(RPN2)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))高(Gao)效(Xiao)氟(Fu)氯(Lu)菊(Ju)酯(Zhi)(标(Biao)准(Zhun)品(Pin)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>98.0%(HPLC)(T)
Clavesin 1蛋(Dan)白(Bai)(CLVS1)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))氟(Fu)氯(Lu)菊(Ju)酯(Zhi)标(Biao)准(Zhun)溶(Rong)液(Ye)(100μg/ml,u=4%,基(Ji)体(Ti):(?)正(Zheng)己(Ji)烷(Wan)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>98.0%(GC)
Clavesin 2蛋(Dan)白(Bai)(CLVS2)(酶(Mei)联(Lian)免(Mian)疫(Yi)吸(Xi)附(Fu)试(Shi)验(Yan)法(Fa))氟(Fu)氯(Lu)菊(Ju)酯(Zhi)标(Biao)准(Zhun)溶(Rong)液(Ye)(10μg/ml,u=5%,基(Ji)体(Ti):(?)正(Zheng)己(Ji)烷(Wan)) 质(Zhi)量(Liang)规(Gui)格(Ge):(?)>99.0%(GC)