【(?)产(Chan)品(Pin)简(Jian)介(Jie)】(?)
中(Zhong)文(Wen)名(Ming)称(Cheng):(?)1,25二(?)羟(Qiang)基(Ji)3(125-(OH)检(Jian)测(Ce)试(Shi)剂(Ji)盒(He)
英(Ying)文(Wen)名(Ming)称(Cheng):(?)1,25 two hydroxy vitamin D3 (1,25- (OH) ELISA kit 2D3/DVD
包(Bao)装(Zhuang)规(Gui)格(Ge):(?)液(Ye)体(Ti)盒(He)装(Zhuang) 96T/48T
保(Bao)存(Cun)条(Tiao)件(Jian):(?)2-8℃(?)((?)不(Bu)使(Shi)用(Yong)时(Shi),(?)部(Bu)分(Fen)试(Shi)剂(Ji)应(Ying)放(Fang)在(Zai)-20℃(?)条(Tiao)件(Jian)下(Xia))(?)。(?)
有(You)效(Xiao)期(Qi):(?)6个(Ge)月(Yue)
存(Cun)储(Chu)运(Yun)输(Shu):(?)低(Di)温(Wen)避(Bi)光(Guang),(?)请(Qing)勿(Wu)重(Zhong)压(Ya),(?)轻(Qing)拿(Na)轻(Qing)放(Fang)((?)现(Xian)货(Huo)供(Gong)应(Ying),(?)一(Yi)般(Ban)为(Wei)快(Kuai)递(Di)运(Yun)输(Shu),(?)江(Jiang)浙(Zhe)沪(Hu)隔(Ge)天(Tian)到(Dao),(?)外(Wai)地(Di)及(Ji)偏(Pian)远(Yuan)地(Di)方(Fang)三(San)至(Zhi)五(Wu)天(Tian)时(Shi)间(Jian)到(Dao)货(Huo)。(?))(?)
可(Ke)测(Ce)种(Zhong)属(Shu):(?)人(Ren)、(?)大(Da)鼠(Shu)、(?)小(Xiao)鼠(Shu)、(?)兔(Tu)子(Zi)、(?)猪(Zhu)、(?)犬(Quan)、(?)猴(Hou)、(?)马(Ma)、(?)牛(Niu)、(?)羊(Yang)、(?)鸡(Ji)、(?)鸭(Ya)、(?)鱼(Yu)等(Deng)。(?)
适(Shi)用(Yong)范(Fan)围(Wei):(?)此(Ci)试(Shi)剂(Ji)盒(He)仅(Jin)用(Yong)于(Yu)科(Ke)研(Yan)实(Shi)验(Yan),(?)禁(Jin)用(Yong)于(Yu)临(Lin)床(Chuang)。(?)
性(Xing)能(Neng)优(You)点(Dian):(?)
1、(?)准(Zhun)确(Que)性(Xing):(?)标(Biao)准(Zhun)品(Pin)线(Xian)性(Xing)回(Hui)归(Gui)与(Yu)预(Yu)期(Qi)浓(Nong)度(Du)相(Xiang)关(Guan)系(Xi)数(Shu)R值(Zhi),(?)大(Da)于(Yu)等(Deng)于(Yu)0.9900。(?)
2、(?)灵(Ling)敏(Min)度(Du):(?)低(Di)检(Jian)测(Ce)浓(Nong)度(Du)小(Xiao)于(Yu)1.0 IU/mL。(?)
3、(?)特(Te)异(Yi)性(Xing):(?)不(Bu)与(Yu)其(Qi)它(Ta)可(Ke)溶(Rong)性(Xing)结(Jie)构(Gou)类(Lei)似(Si)物(Wu)交(Jiao)叉(Cha)反(Fan)应(Ying)。(?)
4、(?)重(Zhong)复(Fu)性(Xing):(?)板(Ban)内(Nei)、(?)板(Ban)间(Jian)变(Bian)异(Yi)系(Xi)数(Shu)均(Jun)小(Xiao)于(Yu)15%。(?)
5、(?)贮(Zhu)藏(Cang):(?)2-8℃(?),(?)避(Bi)光(Guang)防(Fang)潮(Chao)保(Bao)存(Cun)。(?)
6、(?)有(You)效(Xiao)期(Qi):(?)6个(Ge)月(Yue)
7、(?)检(Jian)测(Ce)范(Fan)围(Wei):(?)6.25 IU/mL -200IU/mL
注(Zhu)意(Yi)事(Shi)项(Xiang):(?)
1、(?)试(Shi)剂(Ji)盒(He)从(Cong)冷(Leng)藏(Cang)环(Huan)境(Jing)中(Zhong)取(Qu)出(Chu)应(Ying)在(Zai)室(Shi)温(Wen)平(Ping)衡(Heng)15-30分(Fen)钟(Zhong)后(Hou)方(Fang)可(Ke)使(Shi)用(Yong),(?)酶(Mei)标(Biao)包(Bao)被(Bei)板(Ban)开(Kai)封(Feng)后(Hou)如(Ru)未(Wei)用(Yong)完(Wan),(?)板(Ban)条(Tiao)应(Ying)装(Zhuang)入(Ru)密(Mi)封(Feng)袋(Dai)中(Zhong)保(Bao)存(Cun)。(?)
2、(?)浓(Nong)洗(Xi)涤(Di)液(Ye)可(Ke)能(Neng)会(Hui)有(You)结(Jie)晶(Jing)析(Xi)出(Chu),(?)稀(Xi)释(Shi)时(Shi)可(Ke)在(Zai)水(Shui)浴(Yu)中(Zhong)加(Jia)温(Wen)助(Zhu)溶(Rong),(?)洗(Xi)涤(Di)时(Shi)不(Bu)影(Ying)响(Xiang)结(Jie)果(Guo)。(?)
3、(?)各(Ge)步(Bu)加(Jia)样(Yang)均(Jun)应(Ying)使(Shi)用(Yong)加(Jia)样(Yang)器(Qi),(?)并(Bing)经(Jing)常(Chang)校(Xiao)对(Dui)其(Qi)准(Zhun)确(Que)性(Xing),(?)以(Yi)避(Bi)免(Mian)试(Shi)验(Yan)误(Wu)差(Cha)。(?)一(Yi)次(Ci)加(Jia)样(Yang)时(Shi)间(Jian)控(Kong)制(Zhi)在(Zai)5分(Fen)钟(Zhong)内(Nei),(?)如(Ru)标(Biao)本(Ben)数(Shu)量(Liang)多(Duo),(?)使(Shi)用(Yong)排(Pai)枪(Qiang)加(Jia)样(Yang)。(?)
4、(?)请(Qing)每(Mei)次(Ci)测(Ce)定(Ding)的(De)同(Tong)时(Shi)做(Zuo)标(Biao)准(Zhun)曲(Qu)线(Xian),(?)做(Zuo)复(Fu)孔(Kong)。(?)如(Ru)标(Biao)本(Ben)中(Zhong)待(Dai)测(Ce)物(Wu)质(Zhi)含(Han)量(Liang)过(Guo)高(Gao)(样(Yang)本(Ben)OD值(Zhi)大(Da)于(Yu)标(Biao)准(Zhun)品(Pin)孔(Kong)*孔(Kong)的(De)OD值(Zhi)),(?)请(Qing)先(Xian)用(Yong)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)稀(Xi)释(Shi)一(Yi)定(Ding)倍(Bei)数(Shu)(n倍(Bei))后(Hou)再(Zai)测(Ce)定(Ding),(?)计(Ji)算(Suan)时(Shi)请(Qing)zui后(Hou)乘(Cheng)以(Yi)总(Zong)稀(Xi)释(Shi)倍(Bei)数(Shu)(×n×5)。(?)
5、(?)封(Feng)板(Ban)膜(Mo)只(Zhi)限(Xian)一(Yi)次(Ci)性(Xing)使(Shi)用(Yong),(?)以(Yi)避(Bi)免(Mian)交(Jiao)叉(Cha)污(Wu)染(Ran)。(?)
6、(?)底(Di)物(Wu)请(Qing)避(Bi)光(Guang)保(Bao)存(Cun)。(?)
7、(?)严(Yan)格(Ge)按(An)照(Zhao)说(Shuo)明(Ming)书(Shu)的(De)操(Cao)作(Zuo)进(Jin)行(Xing),(?)试(Shi)验(Yan)结(Jie)果(Guo)判(Pan)定(Ding)必(Bi)须(Xu)以(Yi)酶(Mei)标(Biao)仪(Yi)读(Du)数(Shu)为(Wei)准(Zhun)。(?)
8、(?)所(Suo)有(You)样(Yang)品(Pin),(?)洗(Xi)涤(Di)液(Ye)和(He)各(Ge)种(Zhong)废(Fei)弃(Qi)物(Wu)都(Du)应(Ying)按(An)传(Chuan)染(Ran)物(Wu)处(Chu)理(Li)。(?)
9、(?)本(Ben)试(Shi)剂(Ji)不(Bu)同(Tong)批(Pi)号(Hao)组(Zu)分(Fen)不(Bu)得(De)混(Hun)用(Yong)。(?)
10、(?)如(Ru)与(Yu)英(Ying)文(Wen)说(Shuo)明(Ming)书(Shu)有(You)异(Yi),(?)以(Yi)英(Ying)文(Wen)说(Shuo)明(Ming)书(Shu)为(Wei)准(Zhun)。(?)
ACF1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 溴(Xiu)区(Qu)结(Jie)构(Gou)域(Yu)相(Xiang)邻(Lin)锌(Xin)指(Zhi)蛋(Dan)白(Bai)1A抗(Kang)体(Ti) 0.2ml
Apo-1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 载(Zai)脂(Zhi)蛋(Dan)白(Bai)1抗(Kang)体(Ti) 0.1ml
Amisyn 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 突(Tu)触(Chu)融(Rong)合(He)结(Jie)合(He)蛋(Dan)白(Bai)6抗(Kang)体(Ti) 0.2ml
Arginase 1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 酶(Mei)1抗(Kang)体(Ti) 0.2ml
AKD1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 腺(Xian)苷(Gan)酸(Suan)激(Ji)酶(Mei)结(Jie)构(Gou)域(Yu)蛋(Dan)白(Bai)1抗(Kang)体(Ti) 0.2ml
ABCA7 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 三(San)磷(Lin)酸(Suan)腺(Xian)苷(Gan)结(Jie)合(He)盒(He)转(Zhuan)运(Yun)蛋(Dan)白(Bai)7抗(Kang)体(Ti) 0.2ml
phospho-Na+/K+-ATPase Alpha1(Ser 943) 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 磷(Lin)酸(Suan)化(Hua)钠(Na)钾(Jia)ATP酶(Mei)α1多(Duo)肽(Tai)抗(Kang)体(Ti) 0.2ml
ATP1A1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) Na+/K+-ATPase α1 钠(Na)钾(Jia)ATP酶(Mei)α1抗(Kang)体(Ti) 0.2ml
BAF53b 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 肌(Ji)动(Dong)蛋(Dan)白(Bai)样(Yang)蛋(Dan)白(Bai)6B抗(Kang)体(Ti) 0.2ml
Ataxin 7 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 脊(Ji)髓(Sui)小(Xiao)脑(Nao)共(Gong)济(Ji)失(Shi)调(Diao)蛋(Dan)白(Bai)7抗(Kang)体(Ti) 0.2ml
phospho-c-Abl (Tyr70) 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 磷(Lin)酸(Suan)化(Hua)非(Fei)受(Shou)体(Ti)激(Ji)酶(Mei)c-Abl抗(Kang)体(Ti) 0.1ml
APBA1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) β淀(Dian)粉(Fen)样(Yang)前(Qian)体(Ti)蛋(Dan)白(Bai)结(Jie)合(He)蛋(Dan)白(Bai)1抗(Kang)体(Ti)((?)X11α)(?) 0.1ml
Apolipoprotein E 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 载(Zai)脂(Zhi)蛋(Dan)白(Bai)E抗(Kang)体(Ti) 0.1ml
AFP 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 甲(Jia)胎(Tai)蛋(Dan)白(Bai)AFP抗(Kang)体(Ti) 0.1ml
AE2 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 阴(Yin)离(Li)子(Zi)交(Jiao)换(Huan)蛋(Dan)白(Bai)2抗(Kang)体(Ti) 0.2ml
Aconitase 1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 铁(Tie)调(Diao)节(Jie)蛋(Dan)白(Bai)1抗(Kang)体(Ti) 0.2ml
ATAD3A 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 三(San)磷(Lin)酸(Suan)腺(Xian)苷(Gan)酶(Mei)家(Jia)族(Zu)蛋(Dan)白(Bai)3A抗(Kang)体(Ti) 0.2ml
APOJ 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 载(Zai)脂(Zhi)蛋(Dan)白(Bai)J抗(Kang)体(Ti) 0.1ml
APOH 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 载(Zai)脂(Zhi)蛋(Dan)白(Bai)H抗(Kang)体(Ti) 0.1ml
ART4 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 二(?)磷(Lin)酸(Suan)腺(Xian)苷(Gan)核(He)糖(Tang)基(Ji)转(Zhuan)移(Yi)酶(Mei)4抗(Kang)体(Ti) 0.2ml
AKIRIN2 英(Ying)文(Wen)名(Ming)称(Cheng):(?) AKIRIN2蛋(Dan)白(Bai)抗(Kang)体(Ti) 0.2ml
ARL8B 英(Ying)文(Wen)名(Ming)称(Cheng):(?) ADP核(He)糖(Tang)基(Ji)化(Hua)样(Yang)因(Yin)子(Zi)8B抗(Kang)体(Ti) 0.2ml
ATAD2 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 三(San)磷(Lin)酸(Suan)腺(Xian)苷(Gan)酶(Mei)家(Jia)族(Zu)蛋(Dan)白(Bai)2抗(Kang)体(Ti) 0.2ml
AKIRIN1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) AKIRIN1蛋(Dan)白(Bai)抗(Kang)体(Ti) 0.1ml
1,25二(?)羟(Qiang)基(Ji)3(125-(OH)检(Jian)测(Ce)试(Shi)剂(Ji)盒(He)ASZ1 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 锚(Mao)定(Ding)蛋(Dan)白(Bai)样(Yang)蛋(Dan)白(Bai)1抗(Kang)体(Ti) 0.2ml
Adenylate kinase 2 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 腺(Xian)苷(Gan)酸(Suan)激(Ji)酶(Mei)2抗(Kang)体(Ti) 0.2ml
Acrosin 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 顶(Ding)体(Ti)前(Qian)体(Ti)蛋(Dan)白(Bai)抗(Kang)体(Ti) 0.2ml
AIM1L 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 黑(Hei)色(Se)素(Su)瘤(Liu)缺(Que)失(Shi)样(Yang)蛋(Dan)白(Bai)1抗(Kang)体(Ti) 0.2ml
AER61 英(Ying)文(Wen)名(Ming)称(Cheng):(?) 未(Wei)知(Zhi)糖(Tang)基(Ji)化(Hua)转(Zhuan)移(Yi)酶(Mei)AER61抗(Kang)体(Ti) 0.2ml
操(Cao)作(Zuo)步(Bu)骤(Zhou):(?)
实(Shi)验(Yan)开(Kai)始(Shi)前(Qian),(?)请(Qing)提(Ti)前(Qian)配(Pei)置(Zhi)好(Hao)所(Suo)有(You)试(Shi)剂(Ji),(?)试(Shi)剂(Ji)或(Huo)样(Yang)品(Pin)稀(Xi)释(Shi)时(Shi),(?)均(Jun)需(Xu)混(Hun)匀(Yun),(?)混(Hun)匀(Yun)时(Shi)尽(Jin)量(Liang)避(Bi)免(Mian)起(Qi)泡(Pao)。(?)每(Mei)次(Ci)检(Jian)测(Ce)都(Du)应(Ying)该(Gai)做(Zuo)标(Biao)准(Zhun)曲(Qu)线(Xian)。(?)如(Ru)样(Yang)品(Pin)浓(Nong)度(Du)过(Guo)高(Gao)时(Shi),(?)用(Yong)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)进(Jin)行(Xing)稀(Xi)释(Shi),(?)以(Yi)使(Shi)样(Yang)品(Pin)符(Fu)合(He)试(Shi)剂(Ji)盒(He)的(De)检(Jian)测(Ce)范(Fan)围(Wei)。(?)
1. 加(Jia)样(Yang):(?)分(Fen)别(Bie)设(She)空(Kong)白(Bai)孔(Kong)、(?)标(Biao)准(Zhun)孔(Kong)、(?)待(Dai)测(Ce)样(Yang)品(Pin)孔(Kong)。(?)空(Kong)白(Bai)孔(Kong)加(Jia)样(Yang)品(Pin)稀(Xi)释(Shi)液(Ye)100μl,(?)余(Yu)孔(Kong)分(Fen)别(Bie)加(Jia)标(Biao)准(Zhun)品(Pin)或(Huo)待(Dai)测(Ce)样(Yang)品(Pin)100μl,(?)注(Zhu)意(Yi)不(Bu)要(Yao)有(You)气(Qi)泡(Pao),(?)加(Jia)样(Yang)将(Jiang)样(Yang)品(Pin)加(Jia)于(Yu)酶(Mei)标(Biao)板(Ban)孔(Kong)底(Di)部(Bu),(?)尽(Jin)量(Liang)不(Bu)触(Chu)及(Ji)孔(Kong)壁(Bi),(?)轻(Qing)轻(Qing)晃(Huang)动(Dong)混(Hun)匀(Yun),(?)酶(Mei)标(Biao)板(Ban)加(Jia)上(Shang)盖(Gai)或(Huo)覆(Fu)膜(Mo),(?)37℃(?)反(Fan)应(Ying)120分(Fen)钟(Zhong)。(?)为(Wei)保(Bao)证(Zheng)实(Shi)验(Yan)结(Jie)果(Guo)有(You)效(Xiao)性(Xing),(?)每(Mei)次(Ci)实(Shi)验(Yan)请(Qing)使(Shi)用(Yong)新(Xin)的(De)标(Biao)准(Zhun)品(Pin)溶(Rong)液(Ye)。(?)
2. 弃(Qi)去(Qu)液(Ye)体(Ti),(?)甩(Shuai)干(Gan),(?)不(Bu)用(Yong)洗(Xi)涤(Di)。(?)每(Mei)孔(Kong)加(Jia)标(Biao)记(Ji)抗(Kang)体(Ti)工(Gong)作(Zuo)液(Ye)100μl((?)取(Qu)1μl标(Biao)记(Ji)抗(Kang)体(Ti)加(Jia)99μl标(Biao)记(Ji)抗(Kang)体(Ti)稀(Xi)释(Shi)液(Ye)的(De)比(Bi)例(Li)配(Pei)制(Zhi),(?)轻(Qing)轻(Qing)混(Hun)匀(Yun),(?)在(Zai)使(Shi)用(Yong)前(Qian)一(Yi)小(Xiao)时(Shi)内(Nei)配(Pei)制(Zhi))(?),(?)37℃(?),60分(Fen)钟(Zhong)。(?)
3. 温(Wen)育(Yu)60分(Fen)钟(Zhong)后(Hou),(?)弃(Qi)去(Qu)孔(Kong)内(Nei)液(Ye)体(Ti),(?)甩(Shuai)干(Gan),(?)洗(Xi)板(Ban)3次(Ci),(?)每(Mei)次(Ci)浸(Jin)泡(Pao)1-2分(Fen)钟(Zhong),(?)350μl/每(Mei)孔(Kong),(?)甩(Shuai)干(Gan)。(?)
4. 每(Mei)孔(Kong)加(Jia)辣(La)根(Gen)过(Guo)氧(Yang)化(Hua)物(Wu)酶(Mei)标(Biao)记(Ji)亲(Qin)和(He)素(Su)工(Gong)作(Zuo)液(Ye)((?)同(Tong)标(Biao)记(Ji)抗(Kang)体(Ti)工(Gong)作(Zuo)液(Ye))(?)100μl,(?)37℃(?),(?)60分(Fen)钟(Zhong)。(?)
5. 温(Wen)育(Yu)60分(Fen)钟(Zhong)后(Hou),(?)弃(Qi)去(Qu)孔(Kong)内(Nei)液(Ye)体(Ti),(?)甩(Shuai)干(Gan),(?)洗(Xi)板(Ban)5次(Ci),(?)每(Mei)次(Ci)浸(Jin)泡(Pao)1-2分(Fen)钟(Zhong),(?)350μl/每(Mei)孔(Kong),(?)甩(Shuai)干(Gan)。(?)
6. 依(Yi)序(Xu)每(Mei)孔(Kong)加(Jia)底(Di)物(Wu)溶(Rong)液(Ye)90μl,(?)37℃(?)避(Bi)光(Guang)显(Xian)色(Se)((?)30分(Fen)钟(Zhong)内(Nei),(?)此(Ci)时(Shi)肉(Rou)眼(Yan)可(Ke)见(Jian)标(Biao)准(Zhun)品(Pin)的(De)前(Qian)3-4孔(Kong)有(You)明(Ming)显(Xian)的(De)梯(Ti)度(Du)蓝(Lan)色(Se),(?)后(Hou)3-4孔(Kong)梯(Ti)度(Du)不(Bu)明(Ming)显(Xian),(?)即(Ji)可(Ke)终(Zhong)止(Zhi))(?)。(?)
7. 依(Yi)序(Xu)每(Mei)孔(Kong)加(Jia)终(Zhong)止(Zhi)溶(Rong)液(Ye)50μl,(?)终(Zhong)止(Zhi)反(Fan)应(Ying)((?)此(Ci)时(Shi)蓝(Lan)色(Se)立(Li)转(Zhuan)黄(Huang)色(Se))(?)。(?)终(Zhong)止(Zhi)液(Ye)的(De)加(Jia)入(Ru)顺(Shun)序(Xu)应(Ying)尽(Jin)量(Liang)与(Yu)底(Di)物(Wu)液(Ye)的(De)加(Jia)入(Ru)顺(Shun)序(Xu)相(Xiang)同(Tong)。(?)为(Wei)了(Liao)保(Bao)证(Zheng)实(Shi)验(Yan)结(Jie)果(Guo)的(De)准(Zhun)确(Que)性(Xing),(?)底(Di)物(Wu)反(Fan)应(Ying)时(Shi)间(Jian)到(Dao)后(Hou)应(Ying)尽(Jin)快(Kuai)加(Jia)入(Ru)终(Zhong)止(Zhi)液(Ye)。(?)
8. 用(Yong)酶(Mei)联(Lian)仪(Yi)在(Zai)450nm波(Bo)长(Chang)依(Yi)序(Xu)测(Ce)量(Liang)各(Ge)孔(Kong)的(De)光(Guang)密(Mi)度(Du)((?)OD值(Zhi))(?)。(?) 在(Zai)加(Jia)终(Zhong)止(Zhi)液(Ye)后(Hou)15分(Fen)钟(Zhong)以(Yi)内(Nei)进(Jin)行(Xing)检(Jian)测(Ce)。(?)